cell id cisplatin (fluidigm)

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fluidigm cell id cisplatin
Cell Id Cisplatin, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+id+cisplatin/Cell-ID+Cisplatin-195Pt/pmc13044534-110-7-9
Average 93 stars, based on 52 article reviews

cell id cisplatin - by Bioz Stars, 2026-09

93/100 stars

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Incubation:

Article Title: Evaluation of nanobodies engineered with extra-cysteines for site-specific functionalization.
Article Snippet: Cells were first incubated with Cell-ID Cisplatin (201064, Standard Biotools) at a final concentration of 1 μM for 5 min at room temperature to assess viability. .. Cells were first incubated with Cell-ID Cisplatin (201064, Standard Biotools) at a final concentration of 1 μM for 5 min at room temperature to assess viability. ..

Article Title: Type I interferon signaling promotes mucosal inflammation in murine models of colitis
Article Snippet: Then, cells were stained with fluorophore-conjugated antibodies (Supplemental Table 4) and viability dye (LIVE/DEADTM Fixable Yellow Dead Cell Stain, Thermo Fisher Scientific, L34967) at 4°C for 20 minutes in dark, fixed using BD Cytofix/Cytoperm (BD Biosciences, 554714) at 4°C for 20 minutes in dark, and resuspended in 0.5% BSA in PBS. .. For mass cytometry, cells were first incubated at room temperature with Cell-ID Cisplatin (Standard BioTools) for 20 minutes and then with TruStain FcX (BioLegend) for 10 minutes. .. Then, cells were incubated with metal-conjugated primary antibodies that target surface proteins (Supplemental Table 4) at room temperature for 30 minutes, followed by fixation and permeabilization using Maxpar Fix I buffer for 20 minutes and MaxPar Perm-S buffer (Standard BioTools) for 30 minutes.

Article Title: Latent Neoehrlichia mikurensis Infections May Be Reactivated in Patients With B-Cell Lymphomas Treated With Rituximab.
Article Snippet: .. Next, the cells were washed, incubated with 5 μM Cell ID Cisplatin (Fluidigm, South San Francisco, CA, USA), washed and incubated with an antibody panel against 33 extracellular markers for 30 min at room temperature (RT) (Table S3) and 7% Human TruStain FcX (BioLegend, San Diego, CA, USA). .. The samples were washed, fixed with 1.6% formaldehyde and permeabilised using Foxp3/ Transcription Factor Staining buffer (eBioscience, San Diego, CA, USA) for 60 min at RT prior to a 60 min- incubation at RT with antibodies directed against five intracellular markers (Table S3).

Article Title: Tumour-intrinsic features shape T cell differentiation through precursor to symptomatic multiple myeloma.
Article Snippet: .. Cells were incubated with 5mM Cell-ID Cisplatin (Fluidigm Sciences) in serum free RPMI for 3 minutes at room temperature (rT) to identify dead cells. .. Cells were then washed and incubated with human Fc block (BioLegend) for 10 minutes at rT before being barcoded using 6- choose-3 Cadmium CD45 Live Barcoding (Fluidigm Sciences).

Article Title: Single-cell analysis of signalling and transcriptional responses to type I interferons.
Article Snippet: .. PBMCs were washed in serum-free RPMI and then resuspended at 107 cells/ml in serum-free RPMI containing 0.5 mM Cell-ID Cisplatin (Fluidigm) and incubated at 37 °C for 5 min. .. Cells were washed with RPMI containing 10% (v/v) FCS (Sigma) and 2 mM LGlutamine (R10), centrifuged at 300 × g for 5 min before being resuspended to 6 × 107 cells/ml in R10 and rested at 37 °C for 15 min. 50 μl of cells (3 × 106) cells were transferred to 15 ml falcon © The Author(s) EMBO reports 17 tubes for stimulation and antibody staining.

Concentration Assay:

Mass Cytometry:

Staining:

Article Title: High-Dimensional Protein Analysis Uncovers Distinct Immunologic and Stromal Features in Primary and Metastatic Pancreatic Ductal Adenocarcinoma
Article Snippet: .. Viability was assessed by incubating cells with Cell-ID Cisplatin (Fluidigm Sciences, #201195) in PBS, quenching with Maxpar Cell Staining Buffer, and washing twice. .. Surface and intracellular staining was performed per the manufacturer’s protocol (Fluidigm Sciences) with antibodies listed in Supplementary Table S5.

Article Title: Discovery of natural products that modulate signaling in patient-derived cells
Article Snippet: Briefly, after compound challenge samples were pelleted by centrifugation at 200 × g , resuspended and washed with PBS (HyClone, HyClone Laboratories, Logan, UT), then pelleted, and resuspended in PBS. .. They were then stained with Cell-ID Cisplatin (Fluidigm, South San Francisco, CA) as per the manufacturer's recommended protocol. .. The cells were washed and resuspended in staining medium [CSM: PBS + 1% BSA (Fisher Scientific, Fair Lawn, NJ)].

Article Title: High-dimensional CyTOF profiling reveals distinct maternal and fetal immune landscapes in gestational diabetes mellitus
Article Snippet: .. Cryopreserved PMBCs and CBMCs were thawed in 10% FBS supplemented RPMI-1640 (Gibco, UK) containing 1:10,000 universal nuclease (Thermo Fisher Waltham, MA) and stained with 1.25 uM Cell-ID cisplatin (Standard BioTools) in PBS for 3 min at room temperature. .. Cells were then stained with a cocktail of surface marker antibodies (Table S1) in CyTOF staining buffer (PBS with 0.5% bovine serum albumin [BSA; Sigma] and 0.02% sodium azide [Sigma]) for 30 min at 4°C.

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